Anti-RPA34抗体[9H8 / 34A / alpha SSB34A] (ab81191)

概述

  • 产品名称Anti-RPA34抗体[9H8 / 34A / alpha SSB34A]
  • 描述
    小鼠单克隆抗体[9H8 / 34A / alpha SSB34A] to RPA34
  • 特异性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human RPA34 protein.

  • 阳性对照
    • HeLa cells.
  • 常规说明


    ab81191 inhibits the stimulation of pol alpha, but has no effect on DNA unwinding and pol delta stimulation (Kenny et. al., 1990).

性能

应用

Our Abpromise guarantee covers the use of ab81191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa. For 2 hours at room temperature.
IP Use at 2 µg/mg of lysate. Native verified.
EMSA Use at an assay dependent concentration.

靶标

  • 相关性Replication Protein A (RPA) is a single stranded DNA binding protein. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
  • 细胞定位Nuclear. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • 数据库链接
  • 别名
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • replication protein A2 (32kD) antibody
    • replication protein A2, 32kDa antibody
    • RF A protein 2 antibody
    • RP A p32 antibody
    • RPA2 antibody
    • RPA32 antibody
    see all

Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A] 图像

  • anti RPA34 (version of ab81191 containing BSA and Azide) at 1 µg/ml + human cells

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A] (ab81191)参考文献

This product has been referenced in:
  • Kenny MK  et al. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J Biol Chem 265:7693-700 (1990). WB . Read more (PubMed: 2159011) »

See 1 Publication for this product

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