重组
RabMAb

Anti-RPA32/RPA2抗体[EPR2877Y] (ab76420)

概述

  • 产品名称
    Anti-RPA32/RPA2抗体[EPR2877Y]
    参阅全部 RPA32/RPA2 一抗
  • 描述
    兔单克隆抗体[EPR2877Y] to RPA32/RPA2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human RPA32/RPA2 (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • Human breast carcinoma HeLa cell lysate HUVEC lysate NIH3T3 cell lysate C6 cell lysate
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab76420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
IHC-P
  • 应用说明
    IHC-P: 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
    IP: 1/40.
    WB: 1/5000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Is unsuitable for Flow Cyt or ICC.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
      Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
    • 翻译后修饰
      Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
    • 细胞定位
      Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 60S acidic ribosomal protein P1 antibody
      • AA409079 antibody
      • AI325195 antibody
      • AU020965 antibody
      • ik:tdsubc_2g1 antibody
      • M(2)21C antibody
      • MGC137236 antibody
      • OTTHUMP00000004008 antibody
      • p32 antibody
      • p34 antibody
      • RCJMB04_6d17 replication protein A2, 32kDa antibody
      • REPA2 antibody
      • Replication factor A protein 2 antibody
      • Replication protein A 32 kDa subunit antibody
      • Replication protein A 32kDa subunit antibody
      • Replication protein A 34 kDa subunit antibody
      • Replication protein A antibody
      • Replication Protein A2 (32kDa) antibody
      • Replication protein A2 antibody
      • Replication protein A2, 32kDa antibody
      • RF-A protein 2 antibody
      • Rf-A2 antibody
      • RFA antibody
      • RFA2_HUMAN antibody
      • RP-A p32 antibody
      • RP-A p34 antibody
      • RP21C antibody
      • RPA 2 antibody
      • RPA 32 antibody
      • RPA antibody
      • Rpa2 antibody
      • RPA32 antibody
      • RPA34 antibody
      • RpLP1 antibody
      • RpP2 antibody
      • xx:tdsubc_2g1 antibody
      • zgc:109822 antibody
      see all

    图片

    • All lanes : Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HUVEC lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76420 at 1/250 dilution.

    文献

    This product has been referenced in:
    • Bhattacharyya R  et al. Coralyne, a protoberberine alkaloid, causes robust photosenstization of cancer cells through ATR-p38 MAPK-BAX and JAK2-STAT1-BAX pathways. Chem Biol Interact 285:27-39 (2018). Read more (PubMed: 29486184) »
    • Hoffmann S  et al. TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress. J Cell Biol 212:63-75 (2016). Read more (PubMed: 26711499) »

    See all 2 Publications for this product

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (spermatocytes)
    Permeabilization
    No
    Specification
    spermatocytes
    Blocking step
    Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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