RabMAb

Anti-RPA32/RPA2抗体[EPR2876(2)] (ab109084)

概述

  • 产品名称Anti-RPA32/RPA2抗体[EPR2876(2)]
    参阅全部 RPA32/RPA2 一抗
  • 描述
    兔单克隆抗体[EPR2876(2)] to RPA32/RPA2
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human RPA32/RPA2.

  • 阳性对照
    • HeLa, HUVEC, HepG2, and MCF7 cell lysates; Human colon and Human ovary carcinoma tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
      Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
    • 翻译后修饰Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
    • 细胞定位Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 60S acidic ribosomal protein P1 antibody
      • AA409079 antibody
      • AI325195 antibody
      • AU020965 antibody
      • ik:tdsubc_2g1 antibody
      • M(2)21C antibody
      • MGC137236 antibody
      • OTTHUMP00000004008 antibody
      • p32 antibody
      • p34 antibody
      • RCJMB04_6d17 replication protein A2, 32kDa antibody
      • REPA2 antibody
      • Replication factor A protein 2 antibody
      • Replication protein A 32 kDa subunit antibody
      • Replication protein A 32kDa subunit antibody
      • Replication protein A 34 kDa subunit antibody
      • Replication protein A antibody
      • Replication Protein A2 (32kDa) antibody
      • Replication protein A2 antibody
      • Replication protein A2, 32kDa antibody
      • RF-A protein 2 antibody
      • Rf-A2 antibody
      • RFA antibody
      • RFA2_HUMAN antibody
      • RP-A p32 antibody
      • RP-A p34 antibody
      • RP21C antibody
      • RPA 2 antibody
      • RPA 32 antibody
      • RPA antibody
      • Rpa2 antibody
      • RPA32 antibody
      • RPA34 antibody
      • RpLP1 antibody
      • RpP2 antibody
      • xx:tdsubc_2g1 antibody
      • zgc:109822 antibody
      see all

    Anti-RPA32/RPA2 antibody [EPR2876(2)] 图像

    • All lanes : Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa
    • ab109084 at 1/250 dilution staining RPA32/RPA2 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109084 at 1/250 dilution staining RPA32/RPA2 in Human ovary carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)参考文献

    ab109084 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa cells)
    Specification HeLa cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.2% Triton X100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: .5% · Temperature: 22°C
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    提交于 Aug 30 2012

    I am very pleased to hear you would like to accept our offer and test ab109084 inICC/IF. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"