概述

  • 产品名称Anti-RPA32/RPA2抗体
    参阅全部 RPA32/RPA2 一抗
  • 描述
    兔多克隆抗体to RPA32/RPA2
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human replication protein A2, 32 kDa subunit (LocusLink ID 6118).

  • 阳性对照
    • Whole cell extract from HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

性能

应用

Our Abpromise guarantee covers the use of ab10359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
WB 1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • 翻译后修饰Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • 细胞定位Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60S acidic ribosomal protein P1 antibody
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    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Anti-RPA32/RPA2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.
  • IHC image of RPA32/RPA2 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10359, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 32 kDa

    ab10359 detection of RPA32 by Western Blot. Samples: Whole cell extract (75 µg) from asynchronously growing HeLa cells treated with 10 J/m2 UV radiation for the indicated period of time.

    ab10359 detection of RPA32 by Western Blot. Samples: Whole cell extract (75 µg) from asynchronously growing HeLa cells treated with 10 J/m2 UV radiation for the indicated period of time.

Anti-RPA32/RPA2 antibody (ab10359)参考文献

This product has been referenced in:
  • Guervilly JH  et al. Loss of CHK1 function impedes DNA damage-induced FANCD2 monoubiquitination but normalizes the abnormal G2 arrest in Fanconi anemia. Hum Mol Genet 17:679-89 (2008). Read more (PubMed: 18029388) »

See 1 Publication for this product

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