Anti-RPA32/RPA2抗体[2927C5] (ab51499)

概述

  • 产品名称Anti-RPA32/RPA2抗体[2927C5]
    参阅全部 RPA32/RPA2 一抗
  • 描述
    小鼠单克隆抗体[2927C5] to RPA32/RPA2
  • 经测试应用适用于: Flow Cyt, Dot blot, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: C terminal human RPA2.

  • 阳性对照
    • HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab51499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
Dot blot
WB
  • 应用说明Dot: Use at an assay dependent dilution.
    Flow Cyt: Use 0.5µg
    WB: 1/200. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
      Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
    • 翻译后修饰Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
    • 细胞定位Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 60S acidic ribosomal protein P1 antibody
      • AA409079 antibody
      • AI325195 antibody
      • AU020965 antibody
      • ik:tdsubc_2g1 antibody
      • M(2)21C antibody
      • MGC137236 antibody
      • OTTHUMP00000004008 antibody
      • p32 antibody
      • p34 antibody
      • RCJMB04_6d17 replication protein A2, 32kDa antibody
      • REPA2 antibody
      • Replication factor A protein 2 antibody
      • Replication protein A 32 kDa subunit antibody
      • Replication protein A 32kDa subunit antibody
      • Replication protein A 34 kDa subunit antibody
      • Replication protein A antibody
      • Replication Protein A2 (32kDa) antibody
      • Replication protein A2 antibody
      • Replication protein A2, 32kDa antibody
      • RF-A protein 2 antibody
      • Rf-A2 antibody
      • RFA antibody
      • RFA2_HUMAN antibody
      • RP-A p32 antibody
      • RP-A p34 antibody
      • RP21C antibody
      • RPA 2 antibody
      • RPA 32 antibody
      • RPA antibody
      • Rpa2 antibody
      • RPA32 antibody
      • RPA34 antibody
      • RpLP1 antibody
      • RpP2 antibody
      • xx:tdsubc_2g1 antibody
      • zgc:109822 antibody
      see all

    Anti-RPA32/RPA2 antibody [2927C5] 图像

    • Anti-RPA32/RPA2 antibody [2927C5] (ab51499) at 1/200 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      Anti Mouse IgG at 1/2500 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Flow cytometry using ab51499 at 0.5ug. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg)

    Anti-RPA32/RPA2 antibody [2927C5] (ab51499)参考文献

    ab51499 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab51499.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"