RPA14 overexpression 293T裂解液(全细胞) (ab94054)

概述

  • 产品名称RPA14 overexpression 293T裂解液(全细胞)
  • 描述
    RPA14 overexpression裂解液
  • 常规说明ab94054 is a 293T cell transfected lysate in which Human RPA14 has been transiently over-expressed using a pCMV-RPA14 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

应用

Our Abpromise guarantee covers the use of ab94054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

RPA14 overexpression 293T lysate (whole cell) 图像

  • ab94054 at 15 µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RPA14 antibody (ab58317) at 1/500 dilution

    Lane 1 : RPA14 overexpression 293T lysate (whole cell) (ab94054)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

RPA14 overexpression 293T lysate (whole cell) (ab94054)参考文献

ab94054 has not yet been referenced specifically in any publications.

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