概述

  • 产品名称Anti-Rotavirus抗体
    参阅全部 Rotavirus 一抗
  • 描述
    山羊多克隆抗体to Rotavirus
  • 特异性This antibody is specific against ICPs & late structural (virion) antigens. Uninfected cell reactivity is negative against HEp-2 cells and WI-38 cells by indirect immunofluorescence.
  • 经测试应用适用于: ICC/IF, Conjugationmore details
  • 种属反应性
    Reacts (>90%) with Human rotaviruses.
  • 免疫原

    Bovine Nebraska Calf Diarrhea Virus.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明>95% pure.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
Conjugation Use at an assay dependent dilution.

靶标

  • 相关性Rotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
  • 别名
    • Major inner capsid protein VP6 antibody
    • NSP4 antibody
    • VP6 antibody

Anti-Rotavirus antibody (ab20036)参考文献

This product has been referenced in:
  • Yang Y  et al. Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. Sci China Life Sci 54:82-9 (2011). WB . Read more (PubMed: 21104033) »

See 1 Publication for this product

Product Wall

I have been in contact with the lab regarding the issues which you have been experiencing with ab20036. Per the lab, this product does stain the cytoplasma of infected cells, but it is not a strong antibody in IFA. The lab recommends using it at a 1:1...

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This is a polyclonal antibody that was raised using the whole virus, so it reacts with a number of the viral proteins. In ICC/IF, it gives cytoplasmic staining.

Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this antibody.

I can confirm the immunogen used to producethis antibodyis Rotavirus NCDV (Nebraska Calf Diarrhea Virus). I'msor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"