Anti-ROR2抗体[EPR3779] (ab92379)


  • 产品名称Anti-ROR2抗体[EPR3779]
    参阅全部 ROR2 一抗
  • 描述
    兔单克隆抗体[EPR3779] to ROR2
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues of Human ROR2.

  • 阳性对照
    • HeLa, MCF-7, SH-SY5Y and Saos-2 cell lysates, HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Anti-ROR2 antibody (HRP) [EPR3779] (ab202925)



Our Abpromise guarantee covers the use of ab92379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 105 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/500.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation.
    • 疾病相关Brachydactyly B1
      Robinow syndrome autosomal recessive
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
      Contains 1 FZ (frizzled) domain.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 kringle domain.
      Contains 1 protein kinase domain.
    • 发展阶段Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BDB antibody
      • BDB1 antibody
      • Brachydactyly type B antibody
      • EC antibody
      • MGC163394 antibody
      • Neurotrophic tyrosine kinase antibody
      • Neurotrophic tyrosine kinase, receptor related 2 antibody
      • NTRKR2 antibody
      • Receptor tyrosine kinase-like orphan receptor 2 antibody
      • receptor-related 2 antibody
      • ROR2 antibody
      • ROR2_HUMAN antibody
      • Tyrosine protein kinase transmembrane receptor ROR2 antibody
      • Tyrosine-protein kinase transmembrane receptor Ror2 antibody
      see all

    Anti-ROR2 antibody [EPR3779] 图像

    • All lanes : Anti-ROR2 antibody [EPR3779] (ab92379) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit. at 1/2000 dilution

      Predicted band size : 105 kDa
    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling ROR2 with purified ab92379 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • Flow cytometric analysis of Hela cells using ab92379 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    Anti-ROR2 antibody [EPR3779] (ab92379)参考文献

    This product has been referenced in:
    • Shen YL  et al. Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway. Oncol Lett 8:85-90 (2014). WB . Read more (PubMed: 24959224) »
    • Cao L  et al. Polarizing intestinal epithelial cells electrically through Ror2. J Cell Sci 127:3233-9 (2014). WB ; Human . Read more (PubMed: 24928904) »

    See all 3 Publications for this product

    Product Wall

    Application Flow Cytometry
    Sample Human Cell (Mesenchymal stem cell)
    Permeabilization No
    Gating Strategy Live cells using forward and side scatter
    Specification Mesenchymal stem cell
    Preparation Cell harvesting/tissue preparation method: Trypsin edta and cell dissociation buffer (non-enzyme) tried
    Sample buffer: Pbs

    Abcam user community

    Verified customer

    提交于 Apr 15 2015

    Application Western blot
    Sample Human Cell lysate - other (Mesenchymal stem cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 10 µg
    Specification Mesenchymal stem cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    提交于 Apr 15 2015

    Application Flow Cytometry
    Fixation live non-fixed cells
    Permeabilization No
    Sample Human Cell (hESC H9)
    Specification hESC H9
    Gating Strategy Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
    Preparation Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
    Sample buffer: FACS staining buffer

    Dr. Dmitry Shaposhnikov

    Verified customer

    提交于 Oct 31 2013

    Indeed there has been an error with our system when we published this image. I have let our IT department know and they will correct this immediately. Thank you for pointing this error out to us.

    IHC-P ( Immunhistochemistry on paraffin s...

    Read More

    The RabMAbs that we sell on the Abcam website are for research purposes only and have not been evaluated for use in antibody-dependent cellular cytotoxicity. We cannot recommend them for any purpose other than research level WB, IHC, ICC, IP and FC.

    Thank you for contacting us. Rabbits don't have subclasses of IgG, they only produce one type.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293T cells)
    Loading amount 25 µg
    Specification HEK293T cells
    Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    提交于 Jan 18 2013

    Thank you for contacting us.
    ab104839 was generated against amino acids x-y.

    ab93129 was generated against a partial recombinant protein containing the protein sequence spanning 163-514. A his-tag was used in the prot...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (cartilage precursor to endochondral bone)
    Specification cartilage precursor to endochondral bone
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C

    Abcam user community

    Verified customer

    提交于 Jan 19 2011