Anti-ROR alpha抗体- Carboxyterminal end (ab71016)

概述

  • 产品名称
    Anti-ROR alpha抗体- Carboxyterminal end
    参阅全部 ROR alpha 一抗
  • 描述
    兔多克隆抗体to ROR alpha - Carboxyterminal end
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the C terminal region of human ROR alpha.

  • 阳性对照
    • Jurkat cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
ELISA 1/1000.

靶标

  • 功能
    Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.
  • 组织特异性
    Widely expressed in a number of tissues.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 翻译后修饰
    Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
    Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
    Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • fhl2a antibody
    • NR1F1 antibody
    • Nuclear receptor ROR-alpha antibody
    • Nuclear receptor RZR-alpha antibody
    • Nuclear receptor subfamily 1 group F member 1 antibody
    • RAR-related orphan receptor A antibody
    • Retinoid-related orphan receptor-alpha antibody
    • Rora antibody
    • RORA_HUMAN antibody
    • RZR-ALPHA antibody
    • RZRA antibody
    see all

图片

  • Anti-ROR alpha antibody - Carboxyterminal end (ab71016) at 1/300 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab71016 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Cerebellum)
Loading amount
15 µg
Specification
Cerebellum
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 17 2012

Thank you for your prompt answer. I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful. The attachment you have sent to me has the sequence data for human, ...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. The immunogen used to raise this antibody was a synth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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