概述

  • 产品名称Anti-ROR alpha抗体
    参阅全部 ROR alpha 一抗
  • 描述
    兔多克隆抗体to ROR alpha
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T216 of human ROR alpha.

  • 阳性对照
    • A2058 cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab82289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 63 kDa.

靶标

  • 功能Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.
  • 组织特异性Widely expressed in a number of tissues.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 翻译后修饰Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
    Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
    Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • fhl2a antibody
    • NR1F1 antibody
    • Nuclear receptor ROR-alpha antibody
    • Nuclear receptor RZR-alpha antibody
    • Nuclear receptor subfamily 1 group F member 1 antibody
    • RAR-related orphan receptor A antibody
    • Retinoid-related orphan receptor-alpha antibody
    • Rora antibody
    • RORA_HUMAN antibody
    • RZR-ALPHA antibody
    • RZRA antibody
    see all

Anti-ROR alpha antibody 图像

  • Immunofluorescence of HeLa cells labelling ROR alpha with ab82289. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab82289 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ROR alpha immunoreactivity is localized to the nucleus.

  • Anti-ROR alpha antibody (ab82289) at 1/50 dilution + A2058 cell line lysates at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-ROR alpha antibody (ab82289)参考文献

ab82289 has not yet been referenced specifically in any publications.

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