antigen sequence corresponding to C terminal amino acids 49-79 (GRELMGGIGK TMMQSGGTFG TFMAIGMGIR C) of Human ROMO1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence.
Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line.
Belongs to the MGR2 family.
Expression increases in senescent cells.
Information by UniProt
- bA353C18.2 antibody
- C20orf52 antibody
- Mitochondrial targeting GXXXG protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody (ab121379)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121379 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody (ab121379)
ab121379 at 1/250 dilution staining ROMO1 in paraffin-embedded Human duodenum by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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