概述

  • 产品名称Anti-ROM1抗体
    参阅全部 ROM1 一抗
  • 描述
    小鼠多克隆抗体to ROM1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human ROM1 protein (NP_000318.1)

  • 阳性对照
    • Human kidney lysate; ROM1 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab72973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.

靶标

  • 功能May function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. It is essential for disk morphogenesis.
  • 组织特异性Retina (photoreceptor). In rim region of ROS (rod outer segment) disks.
  • 疾病相关Defects in ROM1 may cause retinitis pigmentosa (RP) [MIM:268000]; when associated with defects in PRPH2.
  • 序列相似性Belongs to the PRPH2/ROM1 family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Retinal outer segment membrane protein 1 antibody
    • Rod outer segment membrane protein 1 antibody
    • ROM antibody
    • ROM1 antibody
    • ROM1_HUMAN antibody
    • ROSP1 antibody
    • RP7 antibody
    • Tetraspanin 23 antibody
    • Tetraspanin-23 antibody
    • Tspan 23 antibody
    • Tspan-23 antibody
    • TSPAN23 antibody
    see all

Anti-ROM1 antibody 图像

  • Anti-ROM1 antibody (ab72973) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ROM1 antibody (ab72973) at 1/500 dilution

    Lane 1 : ROM1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-ROM1 antibody (ab72973)参考文献

ab72973 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step normal goat serum (NGS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Cow Cell (bovine rom1 ortholog expressin in AD293)
Specification bovine rom1 ortholog expressin in AD293
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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提交于 Aug 12 2013

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