antigen sequence: VLEEEFRWLL HDEVHAVLKQ LQDILKEASL RFTLPGSGTE GPAKQENFIL GSCGTDQVKG VLTLQGDALS QADVNLKMPR NN corresponding to amino acids in Human ROGDI.
- Human liver tissue; ROGDI over-expression HEK293T lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
May act as a positive regulator of cell proliferation.
Belongs to the rogdi family.
Information by UniProt
- FLJ22386 antibody
- KTZS antibody
- Leucine zipper domain protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-ROGDI antibody (ab122686)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122686 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROGDI antibody (ab122686)
ab122686, at 1/200 dilution, staining ROGDI in paraffin-embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-ROGDI antibody (ab122686)
All lanes : Anti-ROGDI antibody (ab122686) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : ROGDI over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"