- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
- 产品名称Anti-ROCK2抗体[EPR7141(B)]
参阅全部 ROCK2 一抗 - 描述兔单克隆抗体[EPR7141(B)] to ROCK2
- 经测试应用适用于:
WB, ICC/IFmore details
不适用于: IHC-P - 种属反应性与反应: Mouse, Rat, Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ROCK2 (C terminal).
- 阳性对照
- HeLa, HEK293, L6, RAW264.7 and A673 cell lysates; Human kidney tissue, Mouse and Rat brain tissue lysate.
- 常规说明
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- 解离常数（KD） KD = 8.70 x 10 -11 M Learn more about KD
- 存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.14% BSA, 40% Glycerol -
Concentration information loading... - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR7141(B)
- 同种型IgG
- 研究领域
相关产品
- Alternative Versions
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab125025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa). | |
| ICC/IF | 1/100 - 1/250. |
靶标
- 功能Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
- 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. - 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
- 细胞定位Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
- Information by UniProt
-
数据库链接
- Entrez Gene: 9475 Human
- Entrez Gene: 19878 Mouse
- Entrez Gene: 25537 Rat
- Omim: 604002 Human
- SwissProt: O75116 Human
- SwissProt: P70336 Mouse
- SwissProt: Q62868 Rat
- Unigene: 58617 Human
see all -
别名
- coiled-coil-containing protein kinase 2 antibody
- KIAA0619 antibody
- p164 ROCK 2 antibody
see all
Anti-ROCK2 antibody [EPR7141(B)] 图像
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![Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)](http://www.abcam.cn/ps/products/125/ab125025/Images/ab125025-254639-ab125025KOWB.jpg)
Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)
Predicted band size : 161 kDaLane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ROCK2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A10 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab125025 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab125025 was shown to specifically react with ROCK2 when ROCK2 knockout samples were used. Wild-type and ROCK2 knockout samples were subjected to SDS-PAGE. ab125025 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. -
![Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)](http://www.abcam.cn/ps/products/125/ab125025/Images/ab125025-8-ab125025ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling ROCK2 with purified ab125025 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) primary and ab150120 (AlexaFluor®594 goat anti-mouse) secondary both at 1/1000 dilution. Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and ab150120 (anti-mouse) secondary antibody were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by ab150077 (anti-rabbit secondary antibody).
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![Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)](http://www.abcam.cn/ps/products/125/ab125025/Images/ab125025-6-ab125025WB1.jpg)
Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)All lanes : Anti-ROCK2 antibody [EPR7141(B)] (ab125025) at 1/50000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293 whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 161 kDa
Observed band size : 161 kDa
Blocking and Diluting buffer 5% NFDM/TBST -
![Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)](http://www.abcam.cn/ps/products/125/ab125025/Images/ROCK2-Primary-antibodies-ab125025-1.jpg)
Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)All lanes : Anti-ROCK2 antibody [EPR7141(B)] (ab125025) at 1/10000 dilution (unpurified)
Lane 1 : Hela cell lysate
Lane 2 : L6 cell lysate
Lane 3 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 161 kDa -
![Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)](http://www.abcam.cn/ps/products/125/ab125025/Images/ab125025-7-ab125025WB2.jpg)
Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)Anti-ROCK2 antibody [EPR7141(B)] (ab125025) at 1/10000 dilution + RAW264.7 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 161 kDa
Observed band size : 161 kDa
Blocking and Diluting buffer 5% NFDM/TBST -
](http://www.abcam.cn/ps/products/125/ab125025/Images/ROCK2-Primary-antibodies-ab125025-4.jpg)
Other-Anti-ROCK2 antibody [EPR7141(B)](ab125025)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Anti-ROCK2 antibody [EPR7141(B)] (ab125025)参考文献
This product has been referenced in:
- Han Y et al. Noise-induced cochlear F-actin depolymerization is mediated via ROCK2/p-ERM signaling. J Neurochem 133:617-28 (2015). ICC/IF ; Mouse . Read more (PubMed: 25683353) »
- Cai SD et al. MicroRNA-144 inhibits migration and proliferation in rectal cancer by downregulating ROCK-1. Mol Med Rep 12:7396-402 (2015). Read more (PubMed: 26458302) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"