概述

  • 产品名称Anti-ROCK2抗体
    参阅全部 ROCK2 一抗
  • 描述
    小鼠单克隆抗体to ROCK2
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human ROCK2 aa 1279-1388.

性能

应用

Our Abpromise guarantee covers the use of ab56661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 161 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 21147781

靶标

  • 功能Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • coiled-coil-containing protein kinase 2 antibody
    • KIAA0619 antibody
    • p164 ROCK 2 antibody
    • p164 ROCK-2 antibody
    • Rho associated coiled coil containing protein kinase 2 antibody
    • Rho associated protein kinase 2 antibody
    • Rho associated, coiled coil containing protein kinase II antibody
    • Rho kinase 2 antibody
    • Rho-associated antibody
    • Rho-associated protein kinase 2 antibody
    • ROCK 2 antibody
    • Rock II antibody
    • Rock2 antibody
    • ROCK2_HUMAN antibody
    • Rock2m antibody
    • ROK alpha antibody
    • ROKalpha antibody
    see all

Anti-ROCK2 antibody 图像



  • Predicted band size : 161 kDa
  • ICC/IF image of ab56661 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56661, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab56661 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab56661, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab56661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56661, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ROCK2 antibody (ab56661)参考文献

This product has been referenced in:
  • Gentry EG  et al. Rho Kinase Inhibition as a Therapeutic for Progressive Supranuclear Palsy and Corticobasal Degeneration. J Neurosci 36:1316-23 (2016). Read more (PubMed: 26818518) »
  • Calzado-Martín A  et al. Human bone-lineage cell responses to anisotropic Ti6Al4V surfaces are dependent on their maturation state. J Biomed Mater Res A 102:3154-66 (2014). Human . Read more (PubMed: 24136907) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

All the antibodies are well characterized, please choose based on your requirements. e.g polyclonal/ monoclonal, Rabbit/mouse/sheep etc.

The catalogue numbers are:

ab13660, ab45171, ab56661, a...

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Thank you for contacting us.

We unfortunately do not have anti ROCK antibodies available that can be used for functional assays e.g. activation or inhibition of ROCK. I would suggest searching the similar antibody clone in publications. We h...

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Thank you for contacting us. Ab97592 is 89% homologous with ROCK2 and is therefore likely to cross-react. Ab56661 is 59% homologous with ROCK1 and may or may not cross-react. Unfortunately, we have not tested these antibodies for cross-react...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"