Anti-ROC1抗体[EPR6849(B)] (ab124950)


  • 产品名称Anti-ROC1抗体[EPR6849(B)]
    参阅全部 ROC1 一抗
  • 描述
    兔单克隆抗体[EPR6849(B)] to ROC1
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Human
    不与反应: Rat
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminus of Human ROC1.

  • 阳性对照
    • HepG2, Jurkat and HeLa cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
    • 组织特异性Widely expressed.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the RING-box family.
      Contains 1 RING-type zinc finger.
    • 结构域The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BA554C12.1 antibody
      • E3 ubiquitin-protein ligase RBX1 antibody
      • FLJ60363 antibody
      • MGC13357 antibody
      • MGC1481 antibody
      • OTTHUMP00000028983 antibody
      • Protein ZYP antibody
      • Rbx 1 antibody
      • Rbx1 antibody
      • RBX1_HUMAN antibody
      • Regulator of cullins 1 antibody
      • Ring box 1 antibody
      • Ring box 1 E3 ubiquitin protein ligase antibody
      • RING box protein 1 antibody
      • RING finger protein 75 antibody
      • RING finger protein antibody
      • RING-box protein 1 antibody
      • Ringbox protein 1 antibody
      • RNF 75 antibody
      • RNF75 antibody
      • ROC 1 antibody
      • ZYP protein antibody
      see all

    Anti-ROC1 antibody [EPR6849(B)] 图像

    • All lanes : Anti-ROC1 antibody [EPR6849(B)] (ab124950) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 12 kDa
      Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-ROC1 antibody [EPR6849(B)] (ab124950)参考文献

    ab124950 has not yet been referenced specifically in any publications.

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