重组Anti-RBX1抗体[EPR6849(B)] (ab124950)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6849(B)] to RBX1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RBX1抗体[EPR6849(B)]
参阅全部 RBX1 一抗 -
描述
兔单克隆抗体[EPR6849(B)] to RBX1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse不与反应: Rat -
免疫原
Synthetic peptide within Human RBX1 (N terminal). The exact sequence is proprietary.
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阳性对照
- HepG2, Jurkat and HeLa cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.10 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6849(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa). |
靶标
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功能
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. -
组织特异性
Widely expressed. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the RING-box family.
Contains 1 RING-type zinc finger. -
结构域
The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9978 Human
- Entrez Gene: 56438 Mouse
- Omim: 603814 Human
- SwissProt: P62877 Human
- SwissProt: P62878 Mouse
- Unigene: 474949 Human
- Unigene: 29405 Mouse
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别名
- BA554C12.1 antibody
- E3 ubiquitin-protein ligase RBX1 antibody
- FLJ60363 antibody
see all
图片
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All lanes : Anti-RBX1 antibody [EPR6849(B)] (ab124950) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab124950 尚未被引用在任何文献中。