概述

  • 产品名称Anti-ROC1抗体
    参阅全部 ROC1 一抗
  • 描述
    兔多克隆抗体to ROC1
  • 经测试应用适用于: WB, IP, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Zebrafish
  • 免疫原

    Synthetic peptide:

    CPLDNREWEFQKYGH

    conjugated to KLH, corresponding to C terminal amino acids 94-108 of Human ROC1 (NP_055063.1).

  • 阳性对照
    • Human brain (cerebellum) and placenta tissues.

性能

应用

Our Abpromise guarantee covers the use of ab116253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 12 kDa.
IP Use at an assay dependent concentration.
ELISA 1/2000 - 1/10000.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
  • 组织特异性Widely expressed.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • 结构域The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BA554C12.1 antibody
    • E3 ubiquitin-protein ligase RBX1 antibody
    • FLJ60363 antibody
    • MGC13357 antibody
    • MGC1481 antibody
    • OTTHUMP00000028983 antibody
    • Protein ZYP antibody
    • Rbx 1 antibody
    • Rbx1 antibody
    • RBX1_HUMAN antibody
    • Regulator of cullins 1 antibody
    • Ring box 1 antibody
    • Ring box 1 E3 ubiquitin protein ligase antibody
    • RING box protein 1 antibody
    • RING finger protein 75 antibody
    • RING finger protein antibody
    • RING-box protein 1 antibody
    • Ringbox protein 1 antibody
    • RNF 75 antibody
    • RNF75 antibody
    • ROC 1 antibody
    • ZYP protein antibody
    see all

Anti-ROC1 antibody 图像

  • ab116253, at a 1/500 dilution, staining ROC1 in Formalin-fixed, Paraffin-embedded Human brain (cerebellum) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab116253, at a 1/500 dilution, staining ROC1 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ROC1 antibody (ab116253)参考文献

ab116253 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116253.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"