概述

  • 产品名称Anti-Robo1抗体
    参阅全部 Robo1 一抗
  • 描述
    兔多克隆抗体to Robo1
  • 特异性Some batches may cross-react with Robo2 and Robo3. Please see abreview for more details.
  • 经测试应用适用于: IHC-FoFr, ICC/IF, IHC-Fr, IHC-P, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    C-VLGGYERGEDNNE

    conjugated to KLH, corresponding to amino acids 1632-1644 of Human Robo1.

性能

应用

Our Abpromise guarantee covers the use of ab7279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 19609936
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/3000. Detects a band of approximately 181 kDa.
ELISA 1/4000 - 1/16000.
IP 1/2000.

靶标

  • 功能Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
  • 组织特异性Widely expressed, with exception of kidney.
  • 序列相似性Belongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰Ubiquitinated. May be deubiquitinated by USP33.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deleted in U twenty twenty antibody
    • DUTT 1 antibody
    • DUTT1 antibody
    • FLJ21882 antibody
    • H Robo 1 antibody
    • H-Robo-1 antibody
    • hRobo 1 antibody
    • Robo 1 antibody
    • Robo1 antibody
    • ROBO1_HUMAN antibody
    • Roundabout 1 antibody
    • Roundabout axon guidance receptor homolog 1 antibody
    • Roundabout homolog 1 antibody
    • Roundabout homolog1 precurser antibody
    • Roundabout1 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo1 antibody 图像

  •  ab7279 at 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C. An Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab7279 at a 1/2000 dilution staining Robo1 in mouse muscle satellite cells by Immunohistochemistry/ Immunofluorescence, incubated for 12 hours at +4°C. PFA fixed. Blocked with 5% serum for 1 hour at 20°C. Secondary used at 1/500 monoclonal Goat anti-rabbit conjugated to Alexa Fluor 594 (red).

    See Abreview

  • ab7279 staining Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with ab7279 for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.
  • Anti-Robo1 antibody (ab7279) at 1/1000 dilution + Mouse brain lysate at 35 µg

    Secondary
    Goat anti-Rabbit (H7L) at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 181 kDa (why is the actual band size different from the predicted?)

Anti-Robo1 antibody (ab7279)参考文献

This product has been referenced in:

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (lung endothelial cells)
Loading amount 20 µg
Specification lung endothelial cells
Gel Running Conditions Reduced Denaturing (7%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 May 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Loading amount 25 µg
Specification HEK293T cells
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

提交于 Jan 18 2013

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
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Dr. Alain Chedotal

Verified customer

提交于 Oct 15 2012

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

提交于 Oct 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Sciatic nerve)
Specification Sciatic nerve
Fixative Formaldehyde
Permeabilization Yes - 0.25% TX-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jun 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Scatic nerve)
Loading amount 30 µg
Specification Scatic nerve
Gel Running Conditions Reduced Denaturing (8% SDS gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Xinpeng Dun

Verified customer

提交于 Jun 11 2012

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat DRG sections)
Specification Rat DRG sections
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (muscle satellite cell)
Specification muscle satellite cell
Fixative Paraformaldehyde
Permeabilization Yes - 1% Tween-20
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. D Cornelison

Verified customer

提交于 Dec 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse retina)
Specification mouse retina
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 May 15 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"