Anti-Robo1抗体(ab111726)

概述

  • 产品名称Anti-Robo1抗体
    参阅全部 Robo1 一抗
  • 描述
    山羊多克隆抗体to Robo1
  • 特异性ab11726 does not cross react with Robo2 or Robo3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide:

    QKARPAKKQKHQ

    by a Cysteine residue linker, corresponding to internal sequence amino acids 1417-1428 of Mouse Robo1 (NP_062286.2)

  • 阳性对照
    • Mouse Fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab111726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 181 kDa).

靶标

  • 功能Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
  • 组织特异性Widely expressed, with exception of kidney.
  • 序列相似性Belongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰Ubiquitinated. May be deubiquitinated by USP33.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deleted in U twenty twenty antibody
    • DUTT 1 antibody
    • DUTT1 antibody
    • FLJ21882 antibody
    • H Robo 1 antibody
    • H-Robo-1 antibody
    • hRobo 1 antibody
    • Robo 1 antibody
    • Robo1 antibody
    • ROBO1_HUMAN antibody
    • Roundabout 1 antibody
    • Roundabout axon guidance receptor homolog 1 antibody
    • Roundabout homolog 1 antibody
    • Roundabout homolog1 precurser antibody
    • Roundabout1 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo1 antibody 图像

  • Anti-Robo1 antibody (ab111726) at 0.05 µg/ml + Mouse Fetal brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 181 kDa
  • All lanes : Anti-Robo1 antibody (ab111726) at 500 µg/ml

    Lane 1 : Blank
    Lane 2 : HEK293 cells expressing Robo1
    Lane 3 : HEK293 cells expressing Robo2
    Lane 4 : HEK293 cells expressing Robo3

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-goat IgG conjugated to HRP at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 181 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an abreview submitted by Alain Chedotal, Dépt. of Dev, Institut Vision, France

    See Abreview

Anti-Robo1 antibody (ab111726)参考文献

ab111726 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

提交于 Oct 15 2012

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

提交于 Oct 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"