Anti-RNPS1抗体(ab79233)

Abreviews (1) Submit a Question

概述

  • 产品名称
    Anti-RNPS1抗体
  • 描述
    兔多克隆抗体to RNPS1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human RNPS1.

    参阅Abcam的专有抗源政策

    (Peptide available as ab88206.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; A431; U20S; JEG3; MCF7.

性能

应用

Our Abpromise guarantee covers the use of ab79233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能
    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Part of pre- and post-splicing multiprotein mRNP complexes. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Participates in mRNA 3'-end cleavage. Involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰
    Phosphorylated on one or more of the four Ser/Thr residues (Ser-43, Thr-49, Ser-52 or Ser-53). Ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro.
  • 细胞定位
    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • E5.1 antibody
    • MGC117332 antibody
    • RNA binding protein S1 serine rich domain antibody
    • RNA binding protein with serine rich domain 1 antibody
    • RNA-binding protein with serine-rich domain 1 antibody
    • RNPS 1 antibody
    • rnps1 antibody
    • RNPS1_HUMAN antibody
    • SR protein antibody
    • SR related protein LDC2 antibody
    • SR-related protein LDC2 antibody
    see all

图片

  • Western blot - Anti-RNPS1 antibody (ab79233)
    Western blot - Anti-RNPS1 antibody (ab79233)
    All lanes : Anti-RNPS1 antibody (ab79233) at 1 µg/ml

    Lane 1 : : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 47 kDa (possible isoform)


    Exposure time: 5 minutes


    RNA-binding protein with serine-rich domain 1(RNPS1) contains a significant number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. RNPS1 has 3 isoforms of differing molecular weight and the 50 kDa band is thought to represent isoform 1 (34 kDa) in a heavily phosphorylated form. The band of a slightly lower intensity seen at 47 kDa, is thought to represent isoform 2 (31 kDA) also heavily phosphorylated (SwissProt). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-RNPS1 antibody (ab79233)
    Immunocytochemistry/ Immunofluorescence - Anti-RNPS1 antibody (ab79233)
    ICC/IF image of ab79233 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab79233 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab79233？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

1 Review or Q&A

10
Votes: Highest First

Western blot abreview for Anti-RNPS1 antibody

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2A cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
1 µg
Treatment
Doxycyline induced 24 hrs.
Specification
Neuro2A cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Jul 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册