All lanes : Anti-RNPS1 antibody (ab79233) at 1 µg/ml
Lane 1 : : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
SecondaryAll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 50 kDa (
why is the actual band size different from the predicted?)
Additional bands at: 47 kDa (possible isoform)
Exposure time: 5 minutes
RNA-binding protein with serine-rich domain 1(RNPS1) contains a significant number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. RNPS1 has 3 isoforms of differing molecular weight and the 50 kDa band is thought to represent isoform 1 (34 kDa) in a heavily phosphorylated form. The band of a slightly lower intensity seen at 47 kDa, is thought to represent isoform 2 (31 kDA) also heavily phosphorylated (SwissProt).
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.