概述

  • 产品名称Anti-RNF93抗体
    参阅全部 RNF93 一抗
  • 描述
    兔多克隆抗体to RNF93
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Chimpanzee
  • 免疫原

    A 14-22 amino acid region within synthetic peptide: EDVKCQEDQK LQVLLTQIES KKHQVETAFE RLQQELEQQR CLLLARLREL, corresponding to N terminal amino acids 145-194 of Human RNF93

  • 阳性对照
    • HepG2 cell lysate or human kidney or intestine tissue.

性能

应用

Our Abpromise guarantee covers the use of ab24335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

Anti-RNF93 antibody 图像

  • Anti-RNF93 antibody (ab24335) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling RNF93 with ab24335 at 4µg/ml. Epithelial cells of the renal tubule (indicated with arrows) are positively stained.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RNF93 with ab24335 at 4µg/ml. Epithelial cells of the intestinal vilus (indicated with arrows) are positively stained.

Anti-RNF93 antibody (ab24335)参考文献

ab24335 has not yet been referenced specifically in any publications.

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