概述

  • 产品名称Anti-RNF86抗体
    参阅全部 RNF86 一抗
  • 描述
    山羊多克隆抗体to RNF86
  • 经测试应用适用于: IP, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:

    DSGNHCFKVYRYLQ

    , corresponding to C terminal amino acids 731-744 of Human RNF86.

性能

应用

Our Abpromise guarantee covers the use of ab3942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml.
ELISA Use at an assay dependent dilution. Peptide ELISA (antibody detection limit dilution 1:16,000).

靶标

  • 功能May contribute to the alteration of neural cellular mechanisms.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • 结构域The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin protein ligase TRIM2 antibody
    • KIAA0517 antibody
    • RING finger protein 86 antibody
    • RNF86 antibody
    • Trim2 antibody
    • TRIM2_HUMAN antibody
    • Tripartite motif containing 2 antibody
    • Tripartite motif protein 2 antibody
    • Tripartite motif protein TRIM2 antibody
    • Tripartite motif-containing 2 antibody
    • Tripartite motif-containing protein 2 antibody
    see all

Anti-RNF86 antibody 图像

  • Anti-RNF86 antibody (ab3942) at 0.5 µg/ml + Tissue lysates prepared from Rat brain at 35 µg

    Observed band size : 80,85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab3942 at 6.3 µg/ml staining RNF86 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-RNF86 antibody (ab3942)参考文献

ab3942 has not yet been referenced specifically in any publications.

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