概述

性能

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应用

Our Abpromise guarantee covers the use of ab15850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human RNF8 peptide (ab23278).

靶标

  • 功能E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
  • 组织特异性Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the RNF8 family.
    Contains 1 FHA domain.
    Contains 1 RING-type zinc finger.
  • 发展阶段Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.
  • 结构域The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.
  • 翻译后修饰Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
  • 细胞定位Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C3HC4 type zinc finger protein antibody
    • E3 ubiquitin protein ligase RNF8 antibody
    • E3 ubiquitin-protein ligase RNF8 antibody
    • FLJ12013 antibody
    • KIAA0646 antibody
    • Ring finger protein (C3HC4 type) 8 antibody
    • RING finger protein 8 antibody
    • Ring finger protein 8, E3 ubiquitin protein ligase antibody
    • RNF 8 antibody
    • RNF8 antibody
    • RNF8_HUMAN antibody
    • UBC13/UEV interacting ring finger protein antibody
    see all

Anti-RNF8 antibody 图像

  • Anti-RNF8 antibody (ab15850) at 0.1 µg/ml + Lysates prepared from human lung tissues at 35 µg

    Predicted band size : 56 kDa
  • ab15850 staining RNF8 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using NP40, blocked with 5% BSA fo 1 hour at 25°C and then incubated with ab15850 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-goat polyclonal used at a 1/500 dilution.

    See Abreview

Anti-RNF8 antibody (ab15850)参考文献

ab15850 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - NP40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

提交于 Mar 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"