• 产品名称
  • 描述
    山羊多克隆抗体to RNF7
  • 特异性
    ab99284 is expected to recognize isoform 1 (NP_055060.1).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:


    , corresponding to internal sequence amino acids 59-71 of Human RNF7 (NP_055060.1).

  • 阳性对照
    • Human heart lysate



Our Abpromise guarantee covers the use of ab99284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 13 kDa). An approximately 11 kDa band was observed in Human Heart lysates. An additional band of 28 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


  • 功能
    Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
  • 组织特异性
    Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • 结构域
    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • 翻译后修饰
    Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CKBBP 1 antibody
    • CKBBP1 antibody
    • CKII beta binding protein 1 antibody
    • CKII beta-binding protein 1 antibody
    • Rbx 2 antibody
    • Rbx2 antibody
    • RBX2_HUMAN antibody
    • Regulator of cullins 2 antibody
    • RING box protein 2 antibody
    • RING finger protein 7 antibody
    • RING-box protein 2 antibody
    • RNF 7 antibody
    • RNF7 antibody
    • ROC 2 antibody
    • ROC2 antibody
    • SAG antibody
    • Sensitive to apoptosis gene antibody
    • Sensitive to apoptosis gene protein antibody
    • Zinc RING finger protein SAG antibody
    see all


  • Anti-RNF7 antibody (ab99284) at 1 µg/ml + Human heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 13 kDa



ab99284 has not yet been referenced specifically in any publications.


Thank you for your email.

This antibody has not been tested with Drosophila samples so I am sorry we may not be able to confirm its specificity. I however advise you to run a human cell line lysates as positive control - this will help to figu...

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Sorry! the species is untested. So a positive control from human lysates will be a good idea to try.

Thank you for submitting an Abreview of ab99284. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on ...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Embryos)
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

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提交于 Nov 23 2012

Thank you for your email.

As per our email correspondence, you had previously requested a refund which had been issued and was processed on xxx with Credit Note reference xxx. At this point, I do not think that further troubleshooting w...

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I am very pleased to hear you would like to test ab99284 in drosophila. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for drosophila and include this code in the “Addi...

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