概述

  • 产品名称Anti-RNF6抗体
    参阅全部 RNF6 一抗
  • 描述
    兔多克隆抗体to RNF6
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide: NLVETGTLPI LRLAHFFLLN ESDDDDRIRG LTKEQIDNLS TRHYEHNSID, corresponding to amino acids 576-625 of Human RNF6 (NP_005968)

  • 阳性对照
    • 293T cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab80427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with RNF6 peptide (ab195206). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.
  • 组织特异性Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Defects in RNF6 may be a cause of esophageal cancer (ESCR) [MIM:133239]. A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.
  • 序列相似性Belongs to the RNF12 family.
    Contains 1 RING-type zinc finger.
  • 发展阶段Expressed in embryo and adult testis.
  • 细胞定位Nucleus. Cytoplasm. Cell projection > axon. Nucleus > PML body. Localizes to the PML nuclear bodies in Sertoli cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase RNF6 antibody
    • Ring finger protein (C3H2C3 type) 6 antibody
    • Ring finger protein 6 antibody
    • RING H2 protein antibody
    • RING H2 protein RNF6 antibody
    • RNF 6 antibody
    • RNF6 antibody
    • RNF6_HUMAN antibody
    • SPG2 antibody
    see all

Anti-RNF6 antibody 图像

  • Anti-RNF6 antibody (ab80427) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa

Anti-RNF6 antibody (ab80427)参考文献

ab80427 has not yet been referenced specifically in any publications.

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