概述

  • 产品名称Anti-RNF34抗体
    参阅全部 RNF34 一抗
  • 描述
    兔多克隆抗体to RNF34
  • 特异性Expect reactivity with isoform 1 and 2 of RNF34.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • RNF34 transfected lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the given buffer.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.

靶标

  • 功能E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
  • 组织特异性Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • 结构域The RING-type zinc finger is required for the ubiquitination of target proteins.
    The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
  • 翻译后修饰Autoubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • 细胞定位Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARP 1 antibody
    • CARP-1 antibody
    • CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspases-8 and -10-associated RING finger protein 1 antibody
    • E3 ubiquitin-protein ligase RNF34 antibody
    • FYVE-RING finger protein Momo antibody
    • hRFI antibody
    • Human RING finger homologous to inhibitor of apoptosis protein antibody
    • RFI antibody
    • RIF antibody
    • RIFF antibody
    • RING finger protein 34 antibody
    • RING finger protein RIFF antibody
    • RNF34 antibody
    • RNF34_HUMAN antibody
    see all

Anti-RNF34 antibody 图像

  • All lanes : Anti-RNF34 antibody (ab47255) at 1/1000 dilution

    Lane 1 : Empty vector 293T cell lysate
    Lane 2 : RNF34 transfected 293T cell lysate
    Lane 3 : RFFL transfected 293T cell lysate

    Lysates/proteins at 20 µg/ml per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa

Anti-RNF34 antibody (ab47255)参考文献

ab47255 has not yet been referenced specifically in any publications.

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