概述

  • 产品名称
  • 描述
    山羊多克隆抗体to RNF34
  • 经测试应用
    适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    KAGATSMWASCC

    , corresponding to N terminal amino acids 2-13 of Human RNF34 (NP_079402).

  • 阳性对照
    • Human kidney and prostate tissues.

性能

应用

Our Abpromise guarantee covers the use of ab117084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
  • 组织特异性
    Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • 结构域
    The RING-type zinc finger is required for the ubiquitination of target proteins.
    The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
  • 翻译后修饰
    Autoubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • 细胞定位
    Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARP 1 antibody
    • CARP-1 antibody
    • CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspases-8 and -10-associated RING finger protein 1 antibody
    • E3 ubiquitin-protein ligase RNF34 antibody
    • FYVE-RING finger protein Momo antibody
    • hRFI antibody
    • Human RING finger homologous to inhibitor of apoptosis protein antibody
    • RFI antibody
    • RIF antibody
    • RIFF antibody
    • RING finger protein 34 antibody
    • RING finger protein RIFF antibody
    • RNF34 antibody
    • RNF34_HUMAN antibody
    see all

图片

  • Human kidney tissue (formalin-fixed, paraffin-embedded) stained with ab117084 at 3.75µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human prostate tissue (formalin-fixed, paraffin-embedded) stained with ab117084 at 3.75µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

文献

ab117084 has not yet been referenced specifically in any publications.

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