Anti-RNF31/HOIP抗体- ChIP Grade (ab46322)

概述

  • 产品名称Anti-RNF31/HOIP抗体- ChIP Grade
    参阅全部 RNF31/HOIP 一抗
  • 描述
    兔多克隆抗体to RNF31/HOIP - ChIP Grade
  • 经测试应用适用于: ICC/IF, WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human RNF31/HOIP.

    (Peptide available as ab46321.)

  • 阳性对照
    • ab46322 gave a positive signal in Jurkat Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab46322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ChIP
  • 应用说明ChIP: Use at an assay dependent dilution (PMID 19237537.
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/250. Detects a band of approximately 116 kDa (predicted molecular weight: 119 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
    • 组织特异性Expressed in both normal and transformed breast epithelial cell lines.
    • 序列相似性Contains 2 IBR-type zinc fingers.
      Contains 3 RanBP2-type zinc fingers.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • 结构域The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E3 ubiquitin protein ligase RNF31 antibody
      • FLJ10111 antibody
      • FLJ23501 antibody
      • HOIL 1L interacting protein antibody
      • HOIL-1-interacting protein antibody
      • HOIL1L interacting protein antibody
      • HOIP antibody
      • RING finger protein 31 antibody
      • RNF31 antibody
      • RNF31_HUMAN antibody
      • ZIBRA antibody
      • Zinc in between ring finger ubiquitin associated domain antibody
      • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
      see all

    Anti-RNF31/HOIP antibody - ChIP Grade 图像

    • Anti-RNF31/HOIP antibody - ChIP Grade (ab46322) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 119 kDa
      Observed band size : 116 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 105 kDa (possible isoform),50 kDa (possible isoform),60 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab46322 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 cells at 5µg/ml.

    Anti-RNF31/HOIP antibody - ChIP Grade (ab46322)参考文献

    This product has been referenced in:
    • Damgaard RB  et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell 166:1215-1230.e20 (2016). WB ; Mouse . Read more (PubMed: 27523608) »
    • Zhu J  et al. The atypical ubiquitin ligase RNF31 stabilizes estrogen receptor a and modulates estrogen-stimulated breast cancer cell proliferation. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24441041) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Specification Mouse embryonic fibroblasts
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Dr. Paola Marco i Casanova

    Verified customer

    提交于 Mar 28 2014

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
    Specification HEK293 and hTERT-RPE1
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 28 2014

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK 293)
    Loading amount 30 µg
    Specification HEK 293
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"