Anti-RNF31/HOIP抗体(ab125189)

概述

  • 产品名称Anti-RNF31/HOIP抗体
    参阅全部 RNF31/HOIP 一抗
  • 描述
    兔多克隆抗体to RNF31/HOIP
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1022-1072 of Human RNF31/HOIP (NP_060469.4).

  • 阳性对照
    • HeLa whole cell lysate Jurkat whole cell lysate 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab125189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 120 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
    • 组织特异性Expressed in both normal and transformed breast epithelial cell lines.
    • 序列相似性Contains 2 IBR-type zinc fingers.
      Contains 3 RanBP2-type zinc fingers.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • 结构域The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E3 ubiquitin protein ligase RNF31 antibody
      • FLJ10111 antibody
      • FLJ23501 antibody
      • HOIL 1L interacting protein antibody
      • HOIL-1-interacting protein antibody
      • HOIL1L interacting protein antibody
      • HOIP antibody
      • RING finger protein 31 antibody
      • RNF31 antibody
      • RNF31_HUMAN antibody
      • ZIBRA antibody
      • Zinc in between ring finger ubiquitin associated domain antibody
      • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
      see all

    Anti-RNF31/HOIP antibody 图像

    • All lanes : Anti-RNF31/HOIP antibody (ab125189) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 120 kDa


      Exposure time : 3 minutes

    Anti-RNF31/HOIP antibody (ab125189)参考文献

    ab125189 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Sample Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
    Specification HEK293 and hTERT-RPE1
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 31 2014

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Specification Mouse embryonic fibroblasts
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"