概述

  • 产品名称Anti-RNF25抗体
    参阅全部 RNF25 一抗
  • 描述
    小鼠多克隆抗体to RNF25
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human RNF25 protein (NP_071898.2).

  • 阳性对照
    • HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab89281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

靶标

  • 功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • 结构域The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • 翻译后修饰Ubiquitinated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • RING finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

Anti-RNF25 antibody 图像

  • Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml

    Lane 1 : RNF25 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Anti-RNF25 antibody (ab89281)参考文献

ab89281 has not yet been referenced specifically in any publications.

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