Anti-RNF25抗体(ab89281)
Key features and details
- Mouse polyclonal to RNF25
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RNF25抗体
参阅全部 RNF25 一抗 -
描述
小鼠多克隆抗体to RNF25 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human RNF25. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger.
Contains 1 RWD domain. -
结构域
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination. -
翻译后修饰
Ubiquitinated. - Information by UniProt
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数据库链接
- Entrez Gene: 64320 Human
- SwissProt: Q96BH1 Human
- Unigene: 471403 Human
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别名
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
see all
图片
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Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml
Lane 1 : RNF25 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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Datasheet download
文献 (1)
ab89281 被引用在 1 文献中.
- Cho JH et al. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-?B-mediated ERK reactivation. Cell Death Dis 9:587 (2018). PubMed: 29789542