A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration information loading...
Immunogen affinity purified
ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
Use a concentration of 1 µg/ml.
1/10000 - 1/40000.
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
Protein modification; protein ubiquitination.
Contains 1 RING-type zinc finger.
Contains 1 RWD domain.
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
Information by UniProt
E3 ubiquitin protein ligase RNF25 antibody
E3 ubiquitin-protein ligase RNF25 antibody
Western blot - Anti-RNF25 antibody (ab51475)
All lanes :
Anti-RNF25 antibody (ab51475) at 1/1000 dilution
Lane 1 :
HEK293 lysate from
cells transfected with human
Lane 2 :
HEK293 lysate from
cells transfected with mouse RNF25
Lane 3 :
HEK293 lysate from cells transfected with empty vector
Predicted band size:
Observed band size:
54 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-RNF25 antibody (ab51475)
1ug ab51475 was used in immunoprecipitation of 20 µg HEK293
cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated
complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"