产品名称 Anti-RNF25抗体 参阅全部 RNF25 一抗
WB, IP, ELISA more details
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
Use a concentration of 1 µg/ml.
1/10000 - 1/40000.
功能 E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
通路 Protein modification; protein ubiquitination.
序列相似性 Contains 1 RING-type zinc finger. Contains 1 RWD domain.
结构域 The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
Information by UniProt
E3 ubiquitin protein ligase RNF25 antibody
E3 ubiquitin-protein ligase RNF25 antibody
Anti-RNF25 antibody 图像
Western blot - RNF25 antibody (ab51475)
All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution Lane 1 : HEK293 lysate from
cells transfected with human
RNF25 Lane 2 : HEK293 lysate from
cells transfected with mouse RNF25 Lane 3 : HEK293 lysate from cells transfected with empty vector Predicted band size : 51 kDa Observed band size : 54 kDa ( why is the actual band size different from the predicted?)
Immunoprecipitation - RNF25 antibody (ab51475)
1ug ab51475 was used in immunoprecipitation of 20 µg HEK293
cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated
complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.
Anti-RNF25 antibody (ab51475)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"