概述

  • 产品名称Anti-RNF25抗体
    参阅全部 RNF25 一抗
  • 描述
    兔多克隆抗体to RNF25
  • 经测试应用适用于: WB, IP, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids near the C terminus of Human RNF25.

  • 阳性对照
    • Human Brain Cerebellum tissue.

性能

应用

Our Abpromise guarantee covers the use of ab115543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Predicted molecular weight: 51 kDa.
IP Use at an assay dependent concentration.
ELISA 1/10000 - 1/40000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • 结构域The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • 翻译后修饰Ubiquitinated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • RING finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

Anti-RNF25 antibody 图像

  • ab115543, at 5µg/ml, staining RNF25 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-RNF25 antibody (ab115543)参考文献

ab115543 has not yet been referenced specifically in any publications.

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