Full length human RNF220 protein (AAH98300.1)
- RNF220 transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 10 µg/ml.
||1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 63 kDa).
RNF220 contains one RING-type zinc finger. There are two named isoforms.
- C1orf164 antibody
- Chromosome 1 open reading frame 164 antibody
- FLJ10597 antibody
Western blot - Anti-RNF220 antibody (ab69357)
All lanes :
Anti-RNF220 antibody (ab69357) at 1/500 dilutionLane 1 :
RNF220 transfected 293T cell lysateLane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.SecondaryAll lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size:
63 kDaObserved band size:
80 kDa (why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-RNF220 antibody (ab69357)
ab69357 at 10µg/ml staining RNF220 in Hela cells by ICC/IF.
has not yet been referenced specifically in any publications.
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