Rat, Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to a region between residues 50 and 100 of human RNF22 (NP_006449.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
ab74190 was affinity purified using an epitope specific to RNF22 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Expressed in brain, heart, uterus and testis.
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger. Contains 1 filamin repeat. Contains 6 NHL repeats. Contains 1 RING-type zinc finger.
The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Cytoplasm. Early endosome.
Information by UniProt
Brain expressed ring finger antibody
Brain-expressed RING finger protein antibody
Immunoprecipitation - Anti-RNF22 antibody (ab74190)
Detection of RNF22 by Western Blot of Immunprecipitate.
) at 1µg/ml staining RNF22 in HeLa whole cell lysate immunoprecipitated using ab74190 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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