概述

  • 产品名称Anti-RNF20抗体
    参阅全部 RNF20 一抗
  • 描述
    兔多克隆抗体to RNF20
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human RNF20

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells

性能

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应用

Our Abpromise guarantee covers the use of ab70495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
IP Use at 2 µg/mg of lysate.
ICC/IF 1/1000.

靶标

  • 功能Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • 组织特异性Expressed in the normal brain and also in malignant gliomas (at protein level).
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Anti-RNF20 antibody 图像

  • All lanes : Anti-RNF20 antibody (ab70495) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 171 kDa,280 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human RNF20 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70495 at 3 µg/mg lysate. Subsequent RNF20 WB detection was performed using ab70495 at 1 µg/ml.
  • ab70495 (1/1000) detecting RNF20 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.

    See Abreview

Anti-RNF20 antibody (ab70495)参考文献

This product has been referenced in:
  • Buro LJ  et al. Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1). Epigenetics Chromatin 3:16 (2010). WB ; Human . Read more (PubMed: 20825659) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Feb 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"