The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
Use at 2 µg/mg of lysate.
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Expressed in the normal brain and also in malignant gliomas (at protein level).
Protein modification; protein ubiquitination.
Belongs to the BRE1 family. Contains 1 RING-type zinc finger.
Ring finger protein 20 E3 ubiquitin protein ligase antibody
Western blot - RNF20 antibody (ab70495)
All lanes : Anti-RNF20 antibody (ab70495) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Detection of Human RNF20 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70495 at 3 µg/mg lysate. Subsequent RNF20 WB detection was performed using ab70495 at 1 µg/ml.
Immunocytochemistry/ Immunofluorescence - RNF20 antibody (ab70495)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab70495 (1/1000) detecting RNF20 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.
Buro LJ et al. Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1). Epigenetics Chromatin3:16 (2010).
Read more (PubMed: 20825659) »