The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37, 90 kDa (predicted molecular weight: 90 kDa).
E3 ubiquitin-protein ligase (Probable). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Protein modification; protein ubiquitination.
Contains 1 RING-CH-type zinc finger.
The RING-CH-type zinc finger domain is required for E3 ligase activity.