Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (ACTKTLQQSQ PISAGATTTT TAVAPAGGHS GSTECDLECL VCREPYSCPR) of Human RNF186 (NP_061935).
- Transfected 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RNF186 contains a RING type zinc finger, a motif known to be involved in protein-protein and protein-DNA interactions. The specific function of this protein is unknown.
Membrane; Multi pass membrane protein.
- FLJ20225 antibody
- Ring finger protein 186 antibody
- RNF 186 antibody
- RP11 91K11.1 antibody
Western blot - Anti-RNF186 antibody (ab86546)
Anti-RNF186 antibody (ab86546) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
24 kDaObserved band size:
~24 kDa (why is the actual band size different from the predicted?
)Additional bands at:
65 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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