概述

  • 产品名称Anti-RNF186抗体
    参阅全部 RNF186 一抗
  • 描述
    兔多克隆抗体to RNF186
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (ACTKTLQQSQ PISAGATTTT TAVAPAGGHS GSTECDLECL VCREPYSCPR) of Human RNF186 (NP_061935).

  • 阳性对照
    • Transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性RNF186 contains a RING type zinc finger, a motif known to be involved in protein-protein and protein-DNA interactions. The specific function of this protein is unknown.
  • 细胞定位Membrane; Multi pass membrane protein.
  • 数据库链接
  • 别名
    • FLJ20225 antibody
    • Ring finger protein 186 antibody
    • RNF 186 antibody
    • RP11 91K11.1 antibody

Anti-RNF186 antibody 图像

  • Anti-RNF186 antibody (ab86546) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : ~24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

Anti-RNF186 antibody (ab86546)参考文献

ab86546 has not yet been referenced specifically in any publications.

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