Anti-RNF185抗体(ab73118)
Key features and details
- Mouse polyclonal to RNF185
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RNF185抗体 -
描述
小鼠多克隆抗体to RNF185 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human RNF185. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- RNF185 transfected 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, Whole serum, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate -
Concentration information loading...
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纯度
Purified IgA -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73118于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 20 kDa.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 20 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger. -
结构域
The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity. -
细胞定位
Mitochondrion outer membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 91445 Human
- SwissProt: Q96GF1 Human
- Unigene: 517553 Human
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别名
- E3 ubiquitin protein ligase RNF185 antibody
- E3 ubiquitin-protein ligase RNF185 antibody
- FLJ38628 antibody
see all
图片
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All lanes : Anti-RNF185 antibody (ab73118) at 1/500 dilution
Lane 1 : RNF185 transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 20 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab73118 尚未被引用在任何文献中。