Full length human RNF185 protein (NP_689480)
- RNF185 transfected 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: PBS, pH 7.2
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 20 kDa.
RNF185 contains 1 RING-type zinc finger. The exact function of RNF185 remains unknown. There are 2 named isoforms produced by alternative splicing.
Mitochondrion outer membrane; Multi-pass membrane protein.
- E3 ubiquitin protein ligase RNF185 antibody
- FLJ38628 antibody
- Ring finger protein 185 antibody
Western blot - RNF185 antibody (ab73118)
All lanes :
Anti-RNF185 antibody (ab73118) at 1/500 dilutionLane 1 :
RNF185 transfected 293T cell lysateLane 2 :
non transfected lysate
Lysates/proteins at 25 µg per lane.SecondaryAll lanes :
goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilutionPredicted band size:
20 kDaObserved band size:
21 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"