概述

  • 产品名称Anti-RNF169抗体
    参阅全部 RNF169 一抗
  • 描述
    兔多克隆抗体to RNF169
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 216 - 265 (TETGKRKMDE QKKRDEPLVL KTNLERCPAR LSDSENEEPS RGQMTQTHRS) of human RNF169 (NP_001092108).

  • 阳性对照
    • THP-1 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the RNF169 family.
    Contains 1 RING-type zinc finger.
  • 结构域The MIU motif (motif interacting with ubiquitin) mediates the interaction with both 'Lys-48'- and 'Lys-63'-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
  • 细胞定位Nucleus > nucleoplasm. Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase RNF169 antibody
    • KIAA1991 antibody
    • RING finger protein 169 antibody
    • RN169_HUMAN antibody
    • RNF169 antibody
    see all

Anti-RNF169 antibody 图像

  • Anti-RNF169 antibody (ab87711) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 48 kDa,66 kDa. We are unsure as to the identity of these extra bands.

Anti-RNF169 antibody (ab87711)参考文献

ab87711 has not yet been referenced specifically in any publications.

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