概述

  • 产品名称Anti-RNF144抗体
    参阅全部 RNF144 一抗
  • 描述
    小鼠多克隆抗体to RNF144
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length Human RNF144 (NP_055561.2)

  • 阳性对照
    • Rat brain tissue lysate, HeLa cell lysate, RNF144 transfected 293T cell lysate and HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab89260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.

靶标

  • 功能E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the RBR family. RNF144 subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0161 antibody
    • Probable E3 ubiquitin protein ligase RNF144A antibody
    • Probable E3 ubiquitin-protein ligase RNF144A antibody
    • R144A_HUMAN antibody
    • Ring finger protein 144 antibody
    • RING finger protein 144A antibody
    • RNF 144 antibody
    • RNF 144A antibody
    • rnf144a antibody
    • UBCE7IP4 antibody
    • UbcM4 interacting protein 4 antibody
    • UbcM4-interacting protein 4 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 4 antibody
    • Ubiquitin-conjugating enzyme 7-interacting protein 4 antibody
    see all

Anti-RNF144 antibody 图像

  • Anti-RNF144 antibody (ab89260) at 5 µg/ml + Rat brain tissue lysate at 50 µg

    Predicted band size : 33 kDa
  • Anti-RNF144 antibody (ab89260) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 33 kDa
  • All lanes : Anti-RNF144 antibody (ab89260) at 5 µg/ml

    Lane 1 : RNF144 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 33 kDa
  • ab89260 at 10µg/ml staining RNF144 on HeLa cell by immunofluorescence

Anti-RNF144 antibody (ab89260)参考文献

This product has been referenced in:
  • Ho SR  et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). Read more (PubMed: 24979766) »

See 1 Publication for this product

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