The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
功能E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
通路Protein modification; protein ubiquitination.
序列相似性Contains 1 RING-type zinc finger.
结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
翻译后修饰Auto-ubiquitinated. Phosphorylated upon DNA damage, probably by ATM or ATR.