Anti-RNF130抗体(ab49218)

概述

  • 产品名称Anti-RNF130抗体
    参阅全部 RNF130 一抗
  • 描述
    兔多克隆抗体to RNF130
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    A region within synthetic peptide: ALINVTVQEP GRGAPLTFRI DRGRYGLDSP KAEVRGQVLA PLPLHGVADH, corresponding to N terminal amino acids 37-86 of Human RNF130

  • 阳性对照
    • Fetal thymus lysate

性能

应用

Our Abpromise guarantee covers the use of ab49218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

WB Use a concentration of 2.5 mg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性RNF130 contains a RING finger motif and is similar to g1, a Drosophila zinc-finger protein that is expressed in mesoderm and involved in embryonic development. The expression of the mouse counterpart was found to be upregulated in myeloblastic cells following IL3 deprivation, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells.
  • 细胞定位Membrane; single-pass type I membrane protein
  • 数据库链接
  • 别名
    • 2510042A13Rik antibody
    • G1 related zinc finger protein antibody
    • G1rp antibody
    • G1RZFP antibody
    • GOLIATH antibody
    • GP antibody
    • MGC117241 antibody
    • MGC138647 antibody
    • MGC99542 antibody
    • R goliath antibody
    • RING finger protein 130 antibody
    see all

Anti-RNF130 antibody 图像

  • Anti-RNF130 antibody (ab49218) at 2.5 µg/ml + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

Anti-RNF130 antibody (ab49218)参考文献

ab49218 has not yet been referenced specifically in any publications.

Product Wall

The peptide that was used for the immunogen was derived from the range of amino acids 250 - 300 of the human protein. The numbering of the amino acids refers to the sequence at the UniProt database entry for human RNF130, accession Q86XS8:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"