概述

  • 产品名称Anti-RNF13抗体
    参阅全部 RNF13 一抗
  • 描述
    山羊多克隆抗体to RNF13
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: PNGERDYNIANTV, corresponding to C terminal amino acids 369-381 of Human RNF13.

  • 阳性对照
    • Human pancreas.
  • 常规说明Principal Names – RNF13; RZF; ring finger protein 13; RING zinc finger protein. Official Gene Symbol - RNF13. GenBank Accession Number – NP_009213. LocusLink ID - 11342 (human). Gene Ontology terms - peptidase activity; proteolysis and peptidolysis; nucleus.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab4288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. This antibodoidy stained islets but not in ducts and acini of Human normal pancreas. However, in pancreatic ductal adenocarcinoma staining was in the ducts and acini.
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human RNF13 peptide (ab23020).

靶标

  • 功能E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.
  • 组织特异性Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • 结构域The RING-type zinc finger domain is required for E3 ligase activity.
  • 翻译后修饰Auto-ubiquitinated.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase RNF13 antibody
    • FLJ93817 antibody
    • MGC13689 antibody
    • RING finger protein 13 antibody
    • Ring zinc finger protein antibody
    • RNF 13 antibody
    • Rnf13 antibody
    • RNF13_HUMAN antibody
    • RZF antibody
    see all

Anti-RNF13 antibody (ab4288)参考文献

This product has been referenced in:
  • Zhang Q  et al. RNF13: a novel RING-type ubiquitin ligase over-expressed in pancreatic cancer. Cell Res 19:348-57 (2009). WB, IHC-P ; Human . Read more (PubMed: 18794910) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - Triton
Specification U2OS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Formaldehyde
Username

Miss. Chen Avrahami

Verified customer

提交于 May 16 2016

Thank you very much for contacting us regarding ab4288. I am very sorry to hear that you are experiencing difficulty with this antibody. Where did you place the tag on RNF13? It is possible that the tag is interfering with your ability to detect t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"