Anti-RNF128/GRAIL抗体(ab72533)
Key features and details
- Rabbit polyclonal to RNF128/GRAIL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RNF128/GRAIL抗体
参阅全部 RNF128/GRAIL 一抗 -
描述
兔多克隆抗体to RNF128/GRAIL -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Opossum, Orangutan -
免疫原
Synthetic peptide corresponding to Human RNF128/GRAIL aa 227-253.
Sequence:IFYSARRLRNARAQSRKQRQLKADAKK
Database link: NP_919445.1 -
阳性对照
- Human, mouse and rat spleen lysates. Human brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72533于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
Use a concentration of 5 - 10 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 47 kDa). |
IHC-P
Use a concentration of 5 - 10 µg/ml. |
靶标
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功能
E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 PA (protease associated) domain.
Contains 1 RING-type zinc finger. -
结构域
Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain. -
翻译后修饰
Auto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination. -
细胞定位
Endomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7. - Information by UniProt
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数据库链接
- Entrez Gene: 422382 Chicken
- Entrez Gene: 535869 Cow
- Entrez Gene: 79589 Human
- Entrez Gene: 66889 Mouse
- Entrez Gene: 315911 Rat
- Omim: 300439 Human
- SwissProt: Q29RU0 Cow
- SwissProt: Q8TEB7 Human
see all -
别名
- E3 ubiquitin protein ligase RNF128 antibody
- E3 ubiquitin-protein ligase RNF128 antibody
- FLJ23516 antibody
see all
图片
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All lanes : Anti-RNF128/GRAIL antibody (ab72533) at 1 µg/ml
Lane 1 : human spleen lysate
Lane 2 : human spleen lysate with immunising peptide
Lane 3 : mouse spleen lysate
Lane 4 : rat spleen lysate
Predicted band size: 47 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.Endogenous RNF128 / GRAIL is reported to be detected at approximately 66 kD
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Immunohistochemical staining of paraffin-embedded normal human brain tissue using ab72533 at 5 ug/ml (Panel A) and control rabbit Ig at 5 ug/ml (Panel B).
数据表及文件
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Datasheet download
文献 (1)
ab72533 被引用在 1 文献中.
- Bai XS et al. RNF128 Promotes Malignant Behaviors via EGFR/MEK/ERK Pathway in Hepatocellular Carcinoma. Onco Targets Ther 13:10129-10141 (2020). PubMed: 33116595