概述

  • 产品名称Anti-RNF128抗体
    参阅全部 RNF128 一抗
  • 描述
    兔多克隆抗体to RNF128
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Horse, Dog, Human, Chimpanzee, Monkey
    预测可用于: Chicken, Cow, Opossum, Orangutan
  • 免疫原

    Synthetic peptide:

    IFYSARRLRNARAQSRKQRQLKADAKK

    , corresponding to amino acids 227-253 of Human RNF128 (NP_919445.1).

  • 阳性对照
    • Human, mouse and rat spleen lysates. Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab72533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.

靶标

  • 功能E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • 结构域Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
  • 翻译后修饰Auto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination.
  • 细胞定位Endomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin protein ligase RNF128 antibody
    • E3 ubiquitin-protein ligase RNF128 antibody
    • FLJ23516 antibody
    • Gene related to anergy in lymphocytes protein antibody
    • GRAIL antibody
    • RING finger protein 128 antibody
    • RN128_HUMAN antibody
    • RNF128 antibody
    see all

Anti-RNF128 antibody 图像

  • All lanes : Anti-RNF128 antibody (ab72533) at 1 µg/ml

    Lane 1 : human spleen lysate
    Lane 2 : human spleen lysate with immunising peptide
    Lane 3 : mouse spleen lysate
    Lane 4 : rat spleen lysate


    Predicted band size : 47 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical staining of paraffin-embedded normal human brain tissue using ab72533 at 5 ug/ml (Panel A) and control rabbit Ig at 5 ug/ml (Panel B).

Anti-RNF128 antibody (ab72533)参考文献

ab72533 has not yet been referenced specifically in any publications.

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