• 产品名称
  • 描述
    兔多克隆抗体to RNF128
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: HVQSTNESLQ LVNHEANSVA VDVIPHVDNP TFEEDETPNQ ETAVREIKS, corresponding to C terminal amino acids 354-402 of Human RNF128

  • 阳性对照
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab59150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.


  • 功能
    E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • 结构域
    Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
  • 翻译后修饰
    Auto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination.
  • 细胞定位
    Endomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin protein ligase RNF128 antibody
    • E3 ubiquitin-protein ligase RNF128 antibody
    • FLJ23516 antibody
    • Gene related to anergy in lymphocytes protein antibody
    • GRAIL antibody
    • RING finger protein 128 antibody
    • RN128_HUMAN antibody
    • RNF128 antibody
    see all

Anti-RNF128 antibody 图像

  • Predicted band size : 47 kDa
    Western blot analysis of CD4 T cell lysates from WT mice (lane 1) WT mice treated with anti-CD3/CD28 (lane 2) WT mice treated with FK506 (lane 3) WT mice treated with anti-CD3/CD28 and FK506 (lane 4) TRPV1 KO mice (lane 5) TRPV1 KO mice treated with anti-CD3/CD28 (lane 6) TRPV1 KO mice treated with FK506 (lane 7) and TRPV1 KO mice treated with anti-CD3/CD28 and FK506 (lane 8) labeling RNF128 with ab59150 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
  • Anti-RNF128 antibody (ab59150) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa


Anti-RNF128 antibody (ab59150)参考文献

ab59150 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. Unfortunately, the protein sequence was not checked and we have no supporting data other than the actual WB results. Please rest assured that all of our products are guaranteed (Abpromise) to work in species and appli...

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Thank you for your enquiry. Please rest assured that the molecular weight band that we obtained was checked using the SWISSPROT database, as shown below. Therefore, we are sure that the band we obtained was the RNF128 protein band. http://www.ex...

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