概述

  • 产品名称Anti-RNF121抗体
  • 描述
    兔多克隆抗体to RNF121
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: LFSAVTAFVT FRATRKPLVQ TTPRLVYKWF LLIYKISYAT GIVGYMAVMF, corresponding to amino acids 110-159 of Human RNF121

  • 阳性对照
    • Jurkat cell lysate and human intestine tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

靶标

  • 相关性RNF121 protein contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. Three of them are supported by at least two independent transcripts or ESTs, the full length natures of others are not clear.
  • 细胞定位Membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • 4930544L10Rik antibody
    • FLJ11099 antibody
    • ring finger protein 121 antibody

Anti-RNF121 antibody 图像

  • Anti-RNF121 antibody (ab59152) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RNF121 with ab59152 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnifiaction: 400X.

Anti-RNF121 antibody (ab59152)参考文献

ab59152 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59152.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"