概述

  • 产品名称Anti-RNF12抗体
    参阅全部 RNF12 一抗
  • 描述
    兔多克隆抗体to RNF12
  • 特异性ab22813 recognises RNF12.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide derived from a region between residues 530-584 of Human RNF12.

  • 阳性对照
    • Jurkat cell lysate and Human spermatophore tissue sections.

性能

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应用

Our Abpromise guarantee covers the use of ab22813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

靶标

  • 功能E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.
  • 组织特异性Expressed in many tissues.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the RNF12 family.
    Contains 1 RING-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase RLIM antibody
    • LIM domain-interacting RING finger protein antibody
    • R-LIM antibody
    • Renal carcinoma antigen NY-REN-43 antibody
    • RING finger LIM domain-binding protein antibody
    • RING finger protein 12 antibody
    • RLIM antibody
    • RNF12 antibody
    • RNF12_HUMAN antibody
    see all

Anti-RNF12 antibody 图像

  • ab22813 used at 4-8 µg/ml on parffin embeded tissue: Human spermatophore cells with positive label: Epithelial cells (indicated with arrows)
  • Anti-RNF12 antibody (ab22813) at 1 µg/ml + Jurkat cell lysate
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

Anti-RNF12 antibody (ab22813)参考文献

ab22813 has not yet been referenced specifically in any publications.

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