概述

  • 产品名称
  • 描述
    兔多克隆抗体to RNF11
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Rabbit, Guinea pig, Cow, Cat, Human
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 40-89 (SDDISLLHES QSDRASFGEG TEPDQEPPPP YQEQVPVPVY HPTPSQARLA) of Rat RNF11 (XM_001067720).

  • 阳性对照
    • Rat liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab123140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.
  • 组织特异性
    Expressed at low levels in the lung, liver, kidney, pancreas, spleen, prostate, thymus, ovary, small intestine, colon, and peripheral blood lymphocytes, and, at intermediate levels, in the testis, heart, brain and placenta. Highest expression in the skeletal muscle. In the brain, expressed at different levels in several regions: high levels in the amygdala, moderate in the hippocampus and thalamus, low in the caudate and extremely low levels in the corpus callosum (at protein level). Restricted to neurons, enriched in somatodendritic compartments and excluded from white matter (at protein level). In substantia nigra, present in cell bodies and processes of dopaminergic and nondopaminergic cells (at protein level). In Parkinson disease, sequestered in Lewy bodies and neurites. Overexpressed in breast cancer cells, but not detected in the surrounding stroma and weakly, if at all, in normal breast epithelial cells (at protein level). Also expressed in several tumor cell lines.
  • 序列相似性
    Contains 1 RING-type zinc finger.
  • 结构域
    The WW-binding motif mediates interaction with NEDD4.
  • 翻译后修饰
    Ubiquitinated in the presence of SMURF2 and UBE2D1, as well as WWP1.
    Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome.
  • 细胞定位
    Cytoplasm. Nucleus. Predominantly cytoplasmic, when unphosphorylated, and nuclear, when phosphorylated by PKB/AKT1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CGI 123 antibody
    • RING finger protein 11 antibody
    • RNF11 antibody
    • RNF11_HUMAN antibody
    • Sid 1669 antibody
    • SID1669 antibody
    see all

图片

  • Anti-RNF11 antibody (ab123140) at 1 µg/ml + Rat liver lysate at 10 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa

实验方案

文献

This product has been referenced in:
  • Feng H  et al. Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation. Nanoscale Res Lett 11:240 (2016). ICC/IF ; Rat . Read more (PubMed: 27142878) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (HCT116 colon carcinoma)
Specification
HCT116 colon carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 18 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela, HEK293)
Loading amount
30 µg
Specification
Hela, HEK293
Gel Running Conditions
Reduced Denaturing (13,5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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