Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 34-64 of Human RNASEK.
A2058 cell line lysates; Human prostate carcinoma tissue.
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab104094 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 11 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RNASEK is an endoribonuclease which preferentially cleaves ApU and ApG phosphodiester bonds. Hydrolyzes UpU bonds at a lower rate.
Membrane; Multi-pass membrane protein.
Western blot - RNASEK antibody (ab104094)
Anti-RNASEK antibody (ab104094) at 1/100 dilution + A2058 cell line lysates at 35 µg
Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNASEK antibody (ab104094)
ab104094 at 1/50 dilution, staining RNASEK in Human prostate carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"